This week I ran all of the experiments. I inoculated four
TSB tubes on Wednesday the 22nd to work with the cultures the day
after on Thursday. When I came in on Thursday I immediately noticed a difference
among the cell broths (pictured bellow), which was a surprise because all four
tubes were treated exactly the same way. The first tube was occupied by an cloudy
solution. The second tube had some matter at the bottom but the solution itself
was not cloudy. The third and fourth tube had no trace of cell growth and the
solution looked completely unchanged. I became nervous because my plan was to
conduct the DNA extraction protocols using four different broths and now it
seemed like at least two cultures where not going to work for me at all.
However, I began to think critically, if the TSB tubes where
treated the same way there should be no reason why they shouldn’t all have
bacteria. Even though two tubes looked unchanged I was curious enough to still
use them to try and extract the DNA instead of discarding them, and I’m glad I
did.
To determine if the two cultures in question had bacteria I
used the boiling protocol DNA extraction method on all samples and analyzed the
product of the protocol. The results shocked me. I was expecting the two tubes
that seemed unchanged to have no nuclei acid concertation but it turned out
that both tubes in comparison had greater concentrations of nuclei acid and one
even doubled the amount of DNA material found in the two tubes that appeared to
be saturated with bacteria.
Those results gave me the greenlight to use all tubes for
all protocols that evening.
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