Numbers, Numbers, Numbers

“Having had the green light to run all protocols and using all four bacteria cultures I did exactly that and was left drained at the end of that day” – Thursday, March 23^rd

This week was dedicated to analyzing the products of the DNA extraction methods using microvolume UV-Vis spectrophotometer technology. The results are on the tables below and can be overwhelming to look at.

There were four culture broths used, each with a different color cap:

-Green (g)

-White (w)

-Red (r)

-Blue (b).

There was a total of six protocols:

-Boiling (BP)

-TE Boiling (tBP)

-TE Freeze and Thaw (tFT)

-Lysis (L)

-Meat Tenderizer (MT)

-Commercial Kit (C)

Each culture was used to conduct all of the protocols to determine which protocol was the best. Given that there were four cultures, the experiment was conducted four different times technically. Here are the results:

Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
BPg(Blank)
BPg	33.7	ng/µl	1.48	DNA	50
BPg	33.3	ng/µl	1.48	DNA	50
BPg	34	ng/µl	1.49	DNA	50
BPg	34	ng/µl	1.49	DNA	50
BPg	33.7	ng/µl	1.49	DNA	50
BPw(Reblank)
BPw	31.2	ng/µl	1.52	DNA	50
BPw	30.5	ng/µl	1.49	DNA	50
BPw	35.5	ng/µl	1.4	DNA	50
BPw	30.8	ng/µl	1.49	DNA	50
BPw	31.9	ng/µl	1.52	DNA	50
BPr(Reblank)
BPr	77.2	ng/µl	1.57	DNA	50
BPr	76.7	ng/µl	1.56	DNA	50
BPr	77.2	ng/µl	1.57	DNA	50
BPr	76.7	ng/µl	1.57	DNA	50
BPr	80.2	ng/µl	1.57	DNA	50
PBb(Reblank)
BPb	56.6	ng/µl	1.6	DNA	50
BPb	57.7	ng/µl	1.59	DNA	50
BPb	59	ng/µl	1.61	DNA	50
BPb	57.8	ng/µl	1.58	DNA	50
BPb	57.4	ng/µl	1.61	DNA	50
Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
tBPg(Blank)
tBPg	885.5	ng/µl	1.43	DNA	50
tBPg	883.3	ng/µl	1.44	DNA	50
tBPg	968	ng/µl	1.43	DNA	50
tBPg	885	ng/µl	1.44	DNA	50
tBPg	873.8	ng/µl	1.44	DNA	50
tBPw(Reblank)
tBPw	933.8	ng/µl	1.5	DNA	50
tBPw	956.7	ng/µl	1.5	DNA	50
tBPw	949.1	ng/µl	1.5	DNA	50
tBPw	954.4	ng/µl	1.5	DNA	50
tBPw	948.2	ng/µl	1.49	DNA	50
tBPr(Reblank)
tBPr	963	ng/µl	1.56	DNA	50
tBPr	963.8	ng/µl	1.56	DNA	50
tBPr	967.9	ng/µl	1.56	DNA	50
tBPr	968.6	ng/µl	1.56	DNA	50
tBPr	971.5	ng/µl	1.55	DNA	50
tBPb(Reblank)
tBPb	977.2	ng/µl	1.58	DNA	50
tBPb	997.7	ng/µl	1.57	DNA	50
tBPb	987.7	ng/µl	1.57	DNA	50
tBPb	966.8	ng/µl	1.57	DNA	50
tBPb	993.3	ng/µl	1.57	DNA	50
Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
tFTg(Blank)
tFTg	212.4	ng/µl	1.62	DNA	50
tFTg	212.1	ng/µl	1.63	DNA	50
tFTg	206.4	ng/µl	1.58	DNA	50
tFTg	209.1	ng/µl	1.64	DNA	50
tFTg	217.5	ng/µl	1.64	DNA	50
tFTw(Reblank)
tFTw	253.2	ng/µl	1.54	DNA	50
tFTw	249.3	ng/µl	1.54	DNA	50
tFTw	253.2	ng/µl	1.54	DNA	50
tFTw	256.1	ng/µl	1.54	DNA	50
tFTw	254.5	ng/µl	1.54	DNA	50
tFTr(Reblank)
tFTr	170.8	ng/µl	1.53	DNA	50
tFTr	171.8	ng/µl	1.53	DNA	50
tFTr	174.2	ng/µl	1.54	DNA	50
tFTr	171.7	ng/µl	1.53	DNA	50
tFTr	167.6	ng/µl	1.49	DNA	50
tFTb(Reblank)
tFTb	172.6	ng/µl	1.58	DNA	50
tFTb	176.9	ng/µl	1.58	DNA	50
tFTb	175.2	ng/µl	1.57	DNA	50
tFTb	173.8	ng/µl	1.56	DNA	50
tFTb	175.2	ng/µl	1.57	DNA	50
Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
Lg(Blank)
Lg	258.6	ng/µl	1.68	DNA	50
Lg	356.1	ng/µl	1.71	DNA	50
Lg	186.4	ng/µl	1.58	DNA	50
Lg	301.7	ng/µl	1.63	DNA	50
Lg	112.8	ng/µl	1.69	DNA	50
Lw(Reblank)
Lw	261.9	ng/µl	1.57	DNA	50
Lw	263.2	ng/µl	1.57	DNA	50
Lw	317.3	ng/µl	1.57	DNA	50
Lw	302.6	ng/µl	1.52	DNA	50
Lw	263.2	ng/µl	1.58	DNA	50
Lw(Reblank)
Lr	291.3	ng/µl	1.44	DNA	50
Lr	288	ng/µl	1.44	DNA	50
Lr	117.1	ng/µl	1.43	DNA	50
Lr	109.6	ng/µl	1.44	DNA	50
Lr	289.8	ng/µl	1.43	DNA	50
Lb(Reblank)
Lb	324.2	ng/µl	1.55	DNA	50
Lb	239.3	ng/µl	1.55	DNA	50
Lb	317.8	ng/µl	1.53	DNA	50
Lb	339.3	ng/µl	1.54	DNA	50
Lb	354.1	ng/µl	1.55	DNA	50
Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
MTg(Blank)
MTg	200.8	ng/µl	1.68	DNA	50
MTg	209.8	ng/µl	1.68	DNA	50
MTg	203	ng/µl	1.66	DNA	50
MTg	203.4	ng/µl	1.68	DNA	50
MTg	202.5	ng/µl	1.68	DNA	50
MTg(Reblank)
MTw	205.2	ng/µl	1.54	DNA	50
MTw	206.6	ng/µl	1.53	DNA	50
MTw	207.8	ng/µl	1.54	DNA	50
MTw	207.2	ng/µl	1.54	DNA	50
MTw	255.3	ng/µl	1.52	DNA	50
MTr(Reblank)
MTr	107.5	ng/µl	1.51	DNA	50
MTr	117.5	ng/µl	1.41	DNA	50
MTr	116.7	ng/µl	1.53	DNA	50
MTr	106.6	ng/µl	1.59	DNA	50
MTr	113.4	ng/µl	1.53	DNA	50
MTb(Reblank)
MTb	127.6	ng/µl	1.55	DNA	50
MTb	131.4	ng/µl	1.56	DNA	50
MTb	127.6	ng/µl	1.55	DNA	50
MTb	131.1	ng/µl	1.56	DNA	50
MTb	132.4	ng/µl	1.55	DNA	50
MTb	152.1	ng/µl	1.54	DNA	50
Sample ID	Nucleic Acid	Unit	260/280	Sample Type	Factor
Cg(Blank)
Cg	1786.8	ng/µl	1.5	DNA	50
Cg	1810.2	ng/µl	1.45	DNA	50
Cg	1895.7	ng/µl	1.43	DNA	50
Cg	1685	ng/µl	1.44	DNA	50
Cg	1778.2	ng/µl	1.42	DNA	50
Cw(Reblank)
Cw	514	ng/µl	1.46	DNA	50
Cw	516.5	ng/µl	1.46	DNA	50
Cw	519.9	ng/µl	1.46	DNA	50
Cw	515.6	ng/µl	1.45	DNA	50
Cw	521.2	ng/µl	1.44	DNA	50
Cr(Reblank)
Cr	309.9	ng/µl	1.57	DNA	50
Cr	327.8	ng/µl	1.57	DNA	50
Cr	316.4	ng/µl	1.57	DNA	50
Cr	313.2	ng/µl	1.57	DNA	50
Cr	319.6	ng/µl	1.56	DNA	50
Cb(Reblank)
Cb	357.1	ng/µl	1.55	DNA	50
Cb	345	ng/µl	1.56	DNA	50
Cb	345.2	ng/µl	1.56	DNA	50
Cb	344.2	ng/µl	1.56	DNA	50
Cb	348.2	ng/µl	1.56	DNA	50

Next week I’ll work on obtaining averages and plotting the data on graphs which will perhaps make it easier to see what’s happening but from what I can tell the TE Boiling protocols significantly yielded more DNA than any other protocol competing against it. Which is impressive because it’s one of the less elaborate methods to follow.

There are a number of variables that I would like to mention but I’ll do that next week. Until next time, Luis.